LMO2
Gene Ontology Biological Process
Gene Ontology Molecular Function- E-box binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- bHLH transcription factor binding [IPI]
- cofactor binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- E-box binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- bHLH transcription factor binding [IPI]
- cofactor binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
Gene Ontology Cellular Component
MLLT4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
The LIM domain protein Lmo2 binds to AF6, a translocation partner of the MLL oncogene.
The LIM only protein Lmo2 plays an important role in hematopoiesis and leukemogenesis. Lmo2 acts as a bridging molecule between components of hematopoietic gene regulatory protein complexes. We used the yeast two-hybrid system to identify novel Lmo2 interacting proteins and found that the AF6 protein binds to Lmo2. AF6 is a recurrent fusion partner of MLL, the human homolog of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| LMO2 MLLT4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MLLT4 LMO2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID