Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

HiPLA: High-throughput imaging proximity ligation assay.

Serebryannyy LA, Misteli T

Protein-protein interactions are essential for cellular structure and function. To delineate how the intricate assembly of protein interactions contribute to cellular processes in health and disease, new methodologies that are both highly sensitive and can be applied at large scale are needed. Here, we develop HiPLA (high-throughput imaging proximity ligation assay), a method that employs the well-established antibody-based proximity ligation ... [more]

Methods Dec. 15, 2018; 157();80-87 [Pubmed: 30419336]

Throughput

  • High Throughput

Additional Notes

  • Proximity Ligation Assay

Curated By

  • BioGRID