An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Neto1 associates with the NMDA receptor/amyloid precursor protein complex.

Cousins SL, Innocent N, Stephenson FA

Neuropilin tolloid-like 1 (Neto1), is a CUB domain-containing transmembrane protein that was recently identified as a novel component of the NMDA receptor complex. Here, we have investigated the possible association of Neto1 with the amyloid precursor protein (APP)695/GluN1/GluN2A and APP695/GluN1/GluN2B NMDA receptor trafficking complexes that we have previously identified. Neto1(HA) was shown to co-immunoprecipitate with assembled NMDA receptors via GluN2A ... [more]

J. Neurochem. Sep. 01, 2013; 126(5);554-64 [Pubmed: 23621516]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

GRIN2B

Gene Ontology Biological Process

Gene Ontology Molecular Function

N-methyl-D-aspartate selective glutamate receptor activity [IBA]extracellular-glutamate-gated ion channel activity [IBA]glycine binding [IDA]protein binding [IPI]zinc ion binding [ISS]

Gene Ontology Cellular Component

NETO1