An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The binding of Ku antigen to homeodomain proteins promotes their phosphorylation by DNA-dependent protein kinase.

Schild-Poulter C, Pope L, Giffin W, Kochan JC, Ngsee JK, Traykova-Andonova M, Hache RJ

The Ku antigen (70- and 80-kDa subunits) is a regulatory subunit of DNA-dependent protein kinase (DNA-PK) that promotes the recruitment of the catalytic subunit of DNA-PK (DNA-PKcs) to DNA ends and to specific DNA sequences from which the kinase is activated. Ku and DNA-PKcs plays essential roles in double-stranded DNA break repair and V(D)J recombination and have been implicated in ... [more]

J. Biol. Chem. May. 18, 2001; 276(20);16848-56 [Pubmed: 11279128]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

XRCC6

Gene Ontology Biological Process

Gene Ontology Molecular Function

5'-deoxyribose-5-phosphate lyase activity [IMP]DNA binding [NAS]double-stranded DNA binding [TAS]double-stranded telomeric DNA binding [IDA]poly(A) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]transcription regulatory region DNA binding [IDA]

Gene Ontology Cellular Component

HOXD4