BAIT

SLC1A1

Eaac1, Eaat3, REAAC1
solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Rattus norvegicus
PREY

GRIN1

GluN1, NMDAR1, NR1
glutamate receptor, ionotropic, N-methyl D-aspartate 1
GO Process (55)
GO Function (17)
GO Component (18)

Gene Ontology Biological Process

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

N-methyl-D-aspartate receptor-dependent regulation of the glutamate transporter excitatory amino acid carrier 1.

Waxman EA, Baconguis I, Lynch DR, Robinson MB

The neuronal transporter excitatory amino acid carrier 1 (EAAC1) is enriched in perisynaptic regions, where it may regulate synaptic spillover of glutamate. In this study we examined potential interactions between EAAC1 and ionotropic glutamate receptors. N-Methyl-D-aspartate (NMDA) receptor subunits NR1, NR2A, and NR2B, but not the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunit GluR2, were co-immunoprecipitated with EAAC1 from neuron-enriched hippocampal cultures. ... [more]

J. Biol. Chem. Jun. 15, 2007; 282(24);17594-607 [Pubmed: 17459877]

Throughput

  • Low Throughput

Curated By

  • BioGRID