GRIA4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PICK1
Gene Ontology Biological Process
- ATP catabolic process [ISS]
- DNA methylation involved in embryo development [NAS]
- DNA methylation involved in gamete generation [TAS]
- cellular response to decreased oxygen levels [ISS]
- cellular response to glucose starvation [ISS]
- dendritic spine maintenance [ISS]
- dendritic spine organization [ISS]
- glial cell development [ISS]
- long term synaptic depression [ISS]
- monoamine transport [IDA]
- negative regulation of Arp2/3 complex-mediated actin nucleation [ISS]
- neuronal ion channel clustering [TAS]
- positive regulation of receptor internalization [ISS]
- protein kinase C-activating G-protein coupled receptor signaling pathway [IDA]
- protein phosphorylation [ISS]
- receptor clustering [ISS]
- retrograde vesicle-mediated transport, Golgi to ER [NAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function- ATPase activity [ISS]
- Arp2/3 complex binding [ISS]
- G-protein coupled receptor binding [ISS]
- actin filament binding [ISS]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein domain specific binding [ISS]
- protein kinase C binding [ISS]
- receptor binding [ISS]
- ATPase activity [ISS]
- Arp2/3 complex binding [ISS]
- G-protein coupled receptor binding [ISS]
- actin filament binding [ISS]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein domain specific binding [ISS]
- protein kinase C binding [ISS]
- receptor binding [ISS]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
The PDZ proteins PICK1, GRIP, and syntenin bind multiple glutamate receptor subtypes. Analysis of PDZ binding motifs.
Using sequence homology searches, yeast two-hybrid assays and glutathione S-transferase (GST)-pull-down approaches we have identified a series of glutamate receptor subunits that interact differentially with the PDZ proteins GRIP, PICK1, and syntenin. GST-pull-down experiments identified more interactions than detected by yeast two-hybrid assays. We report several receptor-protein interactions, strong ones include: (i) GRIP and syntenin with mGluR7a, mGluR4a, and mGluR6; ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PICK1 GRIA4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID