BAIT

TXNRD2

SELZ, TR, TR-BETA, TR3, TRXR2
thioredoxin reductase 2
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

PSMC3

TBP1
proteasome (prosome, macropain) 26S subunit, ATPase, 3
GO Process (24)
GO Function (4)
GO Component (7)
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Human immunodeficiency virus type 1 Tat binding protein-1 is a transcriptional coactivator specific for TR.

Ishizuka T, Satoh T, Monden T, Shibusawa N, Hashida T, Yamada M, Mori M

The DNA-binding domain of nuclear hormone receptors functions as an interaction interface for other transcription factors. Using the DNA-binding domain of TRbeta1 as bait in the yeast two-hybrid system, we cloned the Tat binding protein-1 that was originally isolated as a protein binding to the human immunodeficiency virus type 1 Tat transactivator. Tat binding protein-1 has subsequently been identified as ... [more]

Mol. Endocrinol. Aug. 01, 2001; 15(8);1329-43 [Pubmed: 11463857]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PSMC3 TXNRD2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
TXNRD2 PSMC3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID