BAIT

RRAS2

TC21
related RAS viral (r-ras) oncogene homolog 2
GO Process (2)
GO Function (2)
GO Component (4)

Gene Ontology Molecular Function

Homo sapiens
PREY

RAF1

CMD1NN, CRAF, NS5, Raf-1, c-Raf
Raf-1 proto-oncogene, serine/threonine kinase
GO Process (30)
GO Function (5)
GO Component (5)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Signal transduction elements of TC21, an oncogenic member of the R-Ras subfamily of GTP-binding proteins.

Movilla N, Crespo P, Bustelo XR

TC21 is a Ras-like GTPase with high oncogenic potential that is found mutated in some human tumors and overexpressed in breast cancer cell lines. We have conducted cellular and biochemical studies in order to understand the role of this protein in signal transduction and to unveil the signaling elements that participate in the TC21 pathway. Using gene transfer experiments, we ... [more]

Oncogene Oct. 21, 1999; 18(43);5860-9 [Pubmed: 10557073]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RRAS2 RAF1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID