BAIT

HSPA1B

Hsp70, Hsp70-1, Hsp70.1, hsp68

heat shock protein 1B

GO Process (6)

GO Function (1)

GO Component (5)

Gene Ontology Biological Process

DNA repair [IMP]

binding of sperm to zona pellucida [IDA]

negative regulation of apoptotic process [IDA]

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]

response to heat [IDA, IMP]

telomere maintenance [IMP]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

cell body [IDA]

intracellular [IDA]

mitochondrial matrix [ISO]

mitochondrion [IDA, ISS]

zona pellucida receptor complex [IDA]

CRISPR Database VEGA MGI Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

PREY

HSP90AB1

90kDa, AL022974, C81438, Hsp84, Hsp84-1, Hsp90, Hspcb

heat shock protein 90 alpha (cytosolic), class B member 1

GO Process (7)

GO Function (13)

GO Component (12)

Gene Ontology Biological Process

cellular response to interleukin-4 [IDA]

negative regulation of neuron apoptotic process [ISO]

placenta development [IMP]

positive regulation of cell size [ISO]

positive regulation of protein binding [ISO]

positive regulation of protein import into nucleus, translocation [ISO]

positive regulation of protein serine/threonine kinase activity [ISO]

Gene Ontology Molecular Function

ATP binding [ISO]
CTP binding [ISO]
GTP binding [ISO]
MHC class II protein complex binding [ISO]
UTP binding [ISO]
dATP binding [ISO]
double-stranded RNA binding [ISO]
ion channel binding [ISO]
kinase binding [ISO]
poly(A) RNA binding [ISO]
protein binding [IPI]
protein kinase binding [ISO]
sulfonylurea receptor binding [ISO]

Gene Ontology Cellular Component

COP9 signalosome [ISO]

apical plasma membrane [ISO]

basolateral plasma membrane [ISO]

brush border membrane [ISO]

cell surface [ISO]

cytoplasm [IDA, ISO]

extracellular vesicular exosome [ISO]

inclusion body [ISO]

intracellular [IDA]

mitochondrion [IDA, ISO]

CRISPR Database VEGA MGI Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Characterization of the protein-protein interactions determining the heat shock protein (hsp90.hsp70.hsp56) heterocomplex.

Czar MJ, Owens-Grillo JK, Dittmar KD, Hutchison KA, Zacharek AM, Leach KL, Deibel MR, Pratt WB

We have reported previously that the three heat shock proteins hsp56, hsp70, and hsp90 exist together in a heterocomplex in human lymphocyte cytosol (Sanchez, E. R., Faber, L. E., Henzel, W. J., and Pratt, W. B. (1990) Biochemistry 29, 5145-5152). All three of these proteins also exist in the native glucocorticoid receptor heterocomplex isolated from WCL2 cell cytosol and we ... [more]

J. Biol. Chem. Apr. 15, 1994; 269(15);11155-61 [Pubmed: 8157642]

Throughput

Low Throughput

Curated By

BioGRID