CGN
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TJP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- adherens junction [IDA]
- apical junction complex [ISO]
- apical part of cell [IDA, ISO]
- apical plasma membrane [IDA]
- apicolateral plasma membrane [IDA]
- basolateral plasma membrane [ISO]
- cell junction [IDA, ISO]
- cell surface [ISO]
- cell-cell adherens junction [IDA]
- cell-cell junction [IDA]
- cytoplasm [IDA, ISO]
- intercalated disc [IDA]
- intercellular canaliculus [IDA]
- membrane [IDA]
- nucleus [IDA]
- plasma membrane [IDA, ISO]
- tight junction [IDA, ISO]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Evidence for a functional interaction between cingulin and ZO-1 in cultured cells.
Cingulin, a protein component of the submembrane plaque of tight junctions (TJ), contains globular and coiled-coil domains and interacts in vitro with several TJ and cytoskeletal proteins, including the PDZ protein ZO-1. Overexpression of Xenopus cingulin in transfected Xenopus A6 cells resulted in the disruption of endogenous ZO-1 localization, suggesting that cingulin functionally interacts with ZO-1. Glutathione S-transferase pull-down experiments ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID