BAIT

NSP6

ORF1ab, ORF1ab-nsp6, SARS-CoV2 nsp6, SARS-CoV-2 nsp6, R1AB_SARS2, PRO_0000449624, GU280_gp01_nsp6
Non-structural protein 6
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

NUP210

GP210, POM210, PSEC0245
nucleoporin 210kDa
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A SARS-CoV-2 - host proximity interactome

Samavarchi-Tehrani P, Abdouni H, Knight JD, Astori A, Samson R, Lin Z, Kim D, Knapp JJ, St- Germain J, Go CD, Larsen B, Wong CJ, Cassonnet P, Demeret C, Jacob Y, Roth FP, Raught B, Gingras A

Viral replication is dependent on interactions between viral polypeptides and host proteins. Identifying virus-host protein interactions can thus uncover unique opportunities for interfering with the virus life cycle via novel drug compounds or drug repurposing. Importantly, many viral-host protein interactions take place at intracellular membranes and poorly soluble organelles, which are difficult to profile using classical biochemical purification approaches. Applying ... [more]

Status: Preliminary Report

Quantitative Score

  • 1.25 [CLSC]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • SAINTexpress (v.3.6.1) was used to identify viral-host proximity interactions and those with an FDR =< 0.01 were considered high confidence. Control-subtracted and length-adjusted spectral counts (CLSC) were calculated and the top viral-host protein interactions are represented by a higher CLSC score.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP6 NUP210
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.99BioGRID
3209530
NSP6 NUP210
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3659767
NSP6 NUP210
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3651078

Curated By

  • BioGRID