An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RGS10 is a selective activator of G alpha i GTPase activity.

Hunt TW, Fields TA, Casey PJ, Peralta EG

Polypeptides that define a protein family termed RGS (for regulators of G-protein signalling) are encoded by the SST2 gene of the yeast Saccharomyces cerevisiae, the EGL-10 gene of the nematode Caenorhabdatis elegans, and several related mammalian genes. Genetic studies in invertebrates and mammalian cell-transfection experiments indicate that RGS proteins negatively regulate signalling pathways involving seven transmembrane receptors and heterotrimeric G ... [more]

Nature Sep. 12, 1996; 383(6596);175-7 [Pubmed: 8774883]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

GNAZ

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein beta/gamma-subunit complex binding [IBA]G-protein coupled serotonin receptor binding [IBA]GTPase activity [IBA]receptor signaling protein activity [TAS]signal transducer activity [IBA]

Gene Ontology Cellular Component

RGS10