PREY

CALR

CRT, HEL-S-99n, RO, SSA, cC1qR
calreticulin
GO Process (33)
GO Function (15)
GO Component (17)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Calnexin, calreticulin, and ERp57 cooperate in disulfide bond formation in human CD1d heavy chain.

Kang SJ, Cresswell P

Members of the CD1 family of membrane glycoproteins can present antigenic lipids to T lymphocytes. Like major histocompatibility complex class I molecules, they form a heterodimeric complex of a heavy chain and beta(2)-microglobulin (beta(2)m) in the endoplasmic reticulum (ER). Binding of lipid antigens, however, takes place in endosomal compartments, similar to class II molecules, and on the plasma membrane. Unlike ... [more]

J. Biol. Chem. Nov. 22, 2002; 277(47);44838-44 [Pubmed: 12239218]

Throughput

  • Low Throughput

Curated By

  • BioGRID