An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A SARS-CoV-2 - host proximity interactome

Samavarchi-Tehrani P, Abdouni H, Knight JD, Astori A, Samson R, Lin Z, Kim D, Knapp JJ, St- Germain J, Go CD, Larsen B, Wong CJ, Cassonnet P, Demeret C, Jacob Y, Roth FP, Raught B, Gingras A

Viral replication is dependent on interactions between viral polypeptides and host proteins. Identifying virus-host protein interactions can thus uncover unique opportunities for interfering with the virus life cycle via novel drug compounds or drug repurposing. Importantly, many viral-host protein interactions take place at intracellular membranes and poorly soluble organelles, which are difficult to profile using classical biochemical purification approaches. Applying ... [more]

Status: Preliminary Report

Quantitative Score

7.05 [CLSC]

Throughput

High Throughput

Additional Notes

BioID
SAINTexpress (v.3.6.1) was used to identify viral-host proximity interactions and those with an FDR =< 0.01 were considered high confidence. Control-subtracted and length-adjusted spectral counts (CLSC) were calculated and the top viral-host protein interactions are represented by a higher CLSC score.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
S DSTN	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	St-Germain JR (2020)	High	28	BioGRID	2806495

Curated By

BioGRID

Interaction Summary

S

DSTN

Gene Ontology Biological Process

Gene Ontology Cellular Component

Proximity Label-MS