An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The proteoglycans aggrecan and Versican form networks with fibulin-2 through their lectin domain binding.

Olin AI, Moergelin M, Sasaki T, Timpl R, Heinegard D, Aspberg A

Aggrecan, versican, neurocan, and brevican are important components of the extracellular matrix in various tissues. Their amino-terminal globular domains bind to hyaluronan, but the function of their carboxyl-terminal globular domains has long remained elusive. A picture is now emerging where the C-type lectin motif of this domain mediates binding to other extracellular matrix proteins. We here demonstrate that aggrecan, versican, ... [more]

J. Biol. Chem. Jan. 12, 2001; 276(2);1253-61 [Pubmed: 11038354]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

VCAN

Gene Ontology Biological Process

Gene Ontology Molecular Function

extracellular matrix structural constituent [IBA]glycosaminoglycan binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component

FBLN2