BAIT

NSP3

ORF1ab, ORF1ab-nsp3, SARS-CoV2 nsp3, SARS-CoV-2 nsp3, R1AB_SARS2, PRO_0000449621, GU280_gp01_nsp3
Non-structural protein 3
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

VIMP

ADO15, SBBI8, SELS, SEPS1, AD-015
VCP-interacting membrane protein
GO Process (22)
GO Function (4)
GO Component (6)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Global BioID-based SARS-CoV-2 proteins proximal interactome unveils novel ties between viral polypeptides and host factors involved in multiple COVID19-associated mechanisms

Estelle M.N. Laurent, Yorgos Sofianatos, Anastassia Komarova, Jean-Pascal Gimeno, Payman Samavarchi Tehrani, Dae-Kyum Kim, Hala Abdouni, Marie Duhamel, Patricia Cassonnet, Jennifer J. Knapp, Da Kuang, Aditya Chawla, Dayag Sheykhkarimli, Ashyad Rayhan, Roujia Li, Oxana Pogoutse, David E. Hill, Michael A. Calderwood, Pascal Falter-Braun, Patrick Aloy, Ulrich Stelzl, Marc Vidal, Anne-Claude Gingras, Georgios A. Pavlopoulos, Sylvie Van Der Werf, Isabelle Fournier, Frederick P. Roth, Michel Salzet, Caroline Demeret, Yves Jacob, Etienne Coyaud

The worldwide SARS-CoV-2 outbreak poses a serious challenge to human societies and economies. SARS-CoV-2 proteins orchestrate complex pathogenic mechanisms that underlie COVID-19 disease. Thus, understanding how viral polypeptides rewire host protein networks enables better-founded therapeutic research. In complement to existing proteomic studies, in this study we define the first proximal interaction network of SARS-CoV-2 proteins, at the whole proteome level ... [more]

Status: Preliminary Report

Throughput

  • High Throughput

Additional Notes

  • BioID
  • To facilitate data interpretation, an arbitrary filter was used to filter out human prey proteins connected to 8 or more viral bait proteins. High confidence interactors had an FDR < 0.01 and were detected by 7 or fewer viral bait proteins.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP3 VIMP
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

High-BioGRID
-

Curated By

  • BioGRID