BAIT

IKBKB

IKK-beta, IKK2, IKKB, IMD15, NFKBIKB
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
GO Process (31)
GO Function (8)
GO Component (5)

Gene Ontology Biological Process

Homo sapiens
PREY

IRS1

HIRS-1
insulin receptor substrate 1
GO Process (25)
GO Function (7)
GO Component (7)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Serine phosphorylation of insulin receptor substrate 1 by inhibitor kappa B kinase complex.

Gao Z, Hwang D, Bataille F, Lefevre M, York D, Quon MJ, Ye J

Insulin resistance contributes importantly to the pathophysiology of type 2 diabetes mellitus. One mechanism mediating insulin resistance may involve the phosphorylation of serine residues in insulin receptor substrate-1 (IRS-1), leading to impairment in the ability of IRS-1 to activate downstream phosphatidylinositol 3-kinase-dependent pathways. Insulin-resistant states and serine phosphorylation of IRS-1 are associated with the activation of the inhibitor kappaB kinase ... [more]

J. Biol. Chem. Dec. 13, 2002; 277(50);48115-21 [Pubmed: 12351658]

Throughput

  • Low Throughput

Curated By

  • BioGRID