BAIT

KRT10

BCIE, BIE, CK10, EHK, K10, KPP
keratin 10
GO Process (1)
GO Function (1)
GO Component (6)
Homo sapiens
PREY

PRKCZ

PKC-ZETA, PKC2, RP11-181G12.1
protein kinase C, zeta
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Inhibition of protein kinase B (PKB) and PKCzeta mediates keratin K10-induced cell cycle arrest.

Paramio JM, Segrelles C, Ruiz S, Jorcano JL

The intermediate filament cytoskeleton is composed of keratins in all epithelial cells and imparts mechanical integrity to these cells. However, beyond this shared function, the functional significance of the carefully regulated tissue- and differentiation-specific expression of the large keratin family of cytoskeletal proteins remains unclear. We recently demonstrated that expression of keratin K10 or K16 may regulate the phosphorylation of ... [more]

Mol. Cell. Biol. Nov. 01, 2001; 21(21);7449-59 [Pubmed: 11585925]

Throughput

  • Low Throughput

Curated By

  • BioGRID