BAIT

MMP2

CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1
matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Alzheimer's Disease Project
Cell Cycle - Senescence Project
Human Papilloma Virus (HPV) Project
Homo sapiens
PREY

LCN2

24p3, MSFI, NGAL, RP11-395P17.6
lipocalin 2
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase.

Kjeldsen L, Johnsen AH, Sengelov H, Borregaard N

A 25-kDa protein was found to be associated with purified human neutrophil gelatinase. Polyclonal antibodies raised against gelatinase not only recognized gelatinase but also this 25-kDa protein. Specific antibodies against the 25-kDa protein were obtained by affinity purification of the gelatinase antibodies. Immunoblotting and immunoprecipitation studies demonstrated the 135-kDa form of gelatinase to be a complex of 92-kDa gelatinase and ... [more]

J. Biol. Chem. May. 15, 1993; 268(14);10425-32 [Pubmed: 7683678]

Throughput

  • Low Throughput

Curated By

  • BioGRID