Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Supervillin associates with androgen receptor and modulates its transcriptional activity.

Ting HJ, Yeh S, Nishimura K, Chang C

Activation of androgen receptor (AR) via androgen in muscle cells has been closely linked to their growth and differentiation. Here, we report the cloning and characterization of supervillin (SV), a 205-kDa actin-binding protein, as an AR coregulator from the skeletal muscle cDNA library. Mammalian two-hybrid and glutathione S-transferase pull-down assays indicate a domain within SV (amino acids 594-1268) can interact ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jan. 22, 2002; 99(2);661-6 [Pubmed: 11792840]

Throughput

  • Low Throughput

Curated By

  • BioGRID