TEK
Gene Ontology Biological Process
- Tie signaling pathway [ISO, TAS]
- angiogenesis [IMP, TAS]
- cell-matrix adhesion [IMP]
- endothelial cell proliferation [IMP]
- heart development [IMP]
- heart trabecula formation [IMP]
- hemopoiesis [IMP]
- intracellular signal transduction [ISO]
- negative regulation of angiogenesis [ISO]
- negative regulation of apoptotic process [IGI]
- negative regulation of endothelial cell apoptotic process [IMP]
- patterning of blood vessels [TAS]
- peptidyl-tyrosine phosphorylation [IDA, ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of actin cytoskeleton reorganization [ISO]
- positive regulation of angiogenesis [ISO]
- positive regulation of cell adhesion [IMP]
- positive regulation of cytokine secretion involved in immune response [IDA]
- positive regulation of endothelial cell migration [ISO, TAS]
- positive regulation of focal adhesion assembly [ISO]
- positive regulation of intracellular signal transduction [ISO]
- positive regulation of peptidyl-serine phosphorylation [IGI]
- positive regulation of phosphatidylinositol 3-kinase activity [ISO]
- positive regulation of phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of protein import into nucleus [IDA]
- positive regulation of protein kinase B signaling [ISO]
- positive regulation of protein phosphorylation [IDA, ISO]
- positive regulation of vascular endothelial growth factor receptor signaling pathway [TAS]
- protein autophosphorylation [ISO]
- protein oligomerization [ISO]
- regulation of NIK/NF-kappaB signaling [IGI]
- regulation of angiogenesis [IMP]
- regulation of cell migration [IDA, IMP]
- regulation of endothelial cell proliferation [TAS]
- regulation of establishment or maintenance of cell polarity [ISO]
- response to estrogen [ISO]
- response to retinoic acid [IDA]
- single organismal cell-cell adhesion [IMP]
- sprouting angiogenesis [ISO]
- substrate adhesion-dependent cell spreading [ISO]
- transmembrane receptor protein tyrosine kinase signaling pathway [ISO]
- vasculogenesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin filament [ISO]
- apical plasma membrane [ISO]
- basal plasma membrane [ISO]
- basolateral plasma membrane [ISO]
- cell surface [ISO]
- cell-cell junction [ISO]
- integral component of membrane [ISM]
- integral component of plasma membrane [ISO]
- membrane raft [ISO]
- microvillus [ISO]
- perinuclear region of cytoplasm [IDA]
- stress fiber [ISO]
GRB2
Gene Ontology Biological Process
- Ras protein signal transduction [TAS]
- aging [ISO]
- anatomical structure formation involved in morphogenesis [IMP]
- branching involved in labyrinthine layer morphogenesis [IMP]
- cell differentiation [IMP]
- cellular response to ionizing radiation [ISO]
- fibroblast growth factor receptor signaling pathway [IGI]
- insulin receptor signaling pathway [ISO]
- positive regulation of actin filament polymerization [IGI]
- positive regulation of reactive oxygen species metabolic process [ISO]
- positive regulation of signal transduction [IDA]
- protein heterooligomerization [ISO]
- receptor internalization [ISO]
- regulation of MAPK cascade [IGI]
- signal transduction in response to DNA damage [ISO]
Gene Ontology Molecular Function- SH3 domain binding [ISO]
- SH3/SH2 adaptor activity [IDA]
- ephrin receptor binding [IPI, ISO]
- epidermal growth factor receptor binding [ISO]
- identical protein binding [ISO]
- insulin receptor substrate binding [ISO]
- neurotrophin TRKA receptor binding [ISO]
- phosphoprotein binding [ISO]
- phosphotyrosine binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI, ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [IPI]
- SH3 domain binding [ISO]
- SH3/SH2 adaptor activity [IDA]
- ephrin receptor binding [IPI, ISO]
- epidermal growth factor receptor binding [ISO]
- identical protein binding [ISO]
- insulin receptor substrate binding [ISO]
- neurotrophin TRKA receptor binding [ISO]
- phosphoprotein binding [ISO]
- phosphotyrosine binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI, ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [IPI]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Identification of Tek/Tie2 binding partners. Binding to a multifunctional docking site mediates cell survival and migration.
The Tek/Tie2 receptor tyrosine kinase plays a pivotal role in vascular and hematopoietic development. To study the signal transduction pathways that are mediated by this receptor, we have used the yeast two-hybrid system to identify signaling molecules that associate with the phosphorylated Tek receptor. Using this approach, we demonstrate that five molecules, Grb2, Grb7, Grb14, Shp2, and the p85 subunit ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TEK GRB2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TEK GRB2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID