An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mitotic phosphorylation of chromosomal protein HMGN1 inhibits nuclear import and promotes interaction with 14.3.3 proteins.

Prymakowska-Bosak M, Hock R, Catez F, Lim JH, Birger Y, Shirakawa H, Lee K, Bustin M

Progression through mitosis is associated with reversible phosphorylation of many nuclear proteins including that of the high-mobility group N (HMGN) nucleosomal binding protein family. Here we use immunofluorescence and in vitro nuclear import studies to demonstrate that mitotic phosphorylation of the nucleosomal binding domain (NBD) of the HMGN1 protein prevents its reentry into the newly formed nucleus in late telophase. ... [more]

Mol. Cell. Biol. Oct. 01, 2002; 22(19);6809-19 [Pubmed: 12215538]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HMGN1 YWHAZ	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Prymakowska-Bosak M (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

HMGN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]

Gene Ontology Cellular Component

YWHAZ