ID3
Gene Ontology Biological Process
- epithelial cell differentiation [IDA]
- heart development [IGI]
- negative regulation of myoblast differentiation [IMP]
- negative regulation of osteoblast differentiation [IGI]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IGI]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation of apoptotic process [ISO]
- regulation of DNA replication [ISO]
- regulation of cell cycle [ISO]
- transcription, DNA-templated [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ID4
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- brain development [IMP]
- cell proliferation [IMP]
- cellular protein localization [IMP]
- central nervous system myelination [IMP]
- cerebral cortex neuron differentiation [IMP]
- fat cell differentiation [IMP]
- hippocampus development [IMP]
- negative regulation of astrocyte differentiation [IGI]
- negative regulation of fat cell differentiation [IMP]
- negative regulation of neuron differentiation [IMP]
- negative regulation of oligodendrocyte differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [ISO]
- neuroblast proliferation [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of osteoblast differentiation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IGI]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
E2A basic-helix-loop-helix transcription factors are negatively regulated by serum growth factors and by the Id3 protein.
Id3, a member of the Id multigene family of dominant negative helix-loop-helix transcription factors, is induced sharply in murine fibroblasts by serum growth factors. To identify relevant targets of Id3 activity, the yeast two-hybrid system was used to identify proteins that dimerize with Id3. Four murine cDNAs were identified in the screen, all of which encode helix-loop-helix proteins: E12, E47, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ID3 ID4 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID