BAIT

NSP6

ORF1ab, ORF1ab-nsp6, SARS-CoV2 nsp6, SARS-CoV-2 nsp6, R1AB_SARS2, PRO_0000449624, GU280_gp01_nsp6
Non-structural protein 6
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

NPLOC4

NPL4
nuclear protein localization 4 homolog (S. cerevisiae)
UPS Project
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A SARS-CoV-2 BioID-based virus-host membrane protein interactome and virus peptide compendium: new proteomics resources for COVID-19 research

St-Germain JR, Astori A, Samavarchi-Tehrani P, Abdouni H, Macwan V, Kim D, Knapp JJ, Roth FP, Gingras A, Raught B

Key steps of viral replication take place at host cell membranes, but the detection of membrane-associated protein-protein interactions using standard affinity-based approaches (e.g. immunoprecipitation coupled with mass spectrometry, IP-MS) is challenging. To learn more about SARS-CoV-2 - host protein interactions that take place at membranes, we utilized a complementary technique, proximity-dependent biotin labeling (BioID). This approach uncovered a virus-host topology ... [more]

Status: Preliminary Report

Quantitative Score

  • 239.0 [SpecSum]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • SAINT analysis was used to identify SARS-CoV-2 viral-host proximity interactions in human HEK-293 cells and those with a Bayesian FDR =< 0.01 were considered high confidence. Scores are the sum of peptide counts from four mass spec runs with a higher score indicating a higher degree of connectivity between proteins.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP6 NPLOC4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3650874
NSP6 NPLOC4
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2800541

Curated By

  • BioGRID