An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Oncoprotein TLS interacts with serine-arginine proteins involved in RNA splicing.

Yang L, Embree LJ, Tsai S, Hickstein DD

The gene encoding the human TLS protein, also termed FUS, is located at the site of chromosomal translocations in human leukemias and sarcomas where it forms a chimeric fusion gene with one of several different genes. To identify interacting partners of TLS, we screened a yeast two-hybrid cDNA library constructed from mouse hematopoietic cells using the C-terminal region of TLS ... [more]

J. Biol. Chem. Oct. 23, 1998; 273(43);27761-4 [Pubmed: 9774382]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FUS SRSF2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yang L (1998)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FUS

Gene Ontology Cellular Component

SRSF2

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [ISO]pre-mRNA binding [IDA]protein binding [IPI]