BAIT

NSP4

ORF1ab, ORF1ab-nsp4, SARS-CoV2 nsp4, SARS-CoV-2 nsp4, R1AB_SARS2, PRO_0000449622, GU280_gp01_nsp4
Non-structural protein 4
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

STOM

BND7, EPB7, EPB72, RP11-342H3.1
stomatin
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A SARS-CoV-2 BioID-based virus-host membrane protein interactome and virus peptide compendium: new proteomics resources for COVID-19 research

St-Germain JR, Astori A, Samavarchi-Tehrani P, Abdouni H, Macwan V, Kim D, Knapp JJ, Roth FP, Gingras A, Raught B

Key steps of viral replication take place at host cell membranes, but the detection of membrane-associated protein-protein interactions using standard affinity-based approaches (e.g. immunoprecipitation coupled with mass spectrometry, IP-MS) is challenging. To learn more about SARS-CoV-2 - host protein interactions that take place at membranes, we utilized a complementary technique, proximity-dependent biotin labeling (BioID). This approach uncovered a virus-host topology ... [more]

Status: Preliminary Report

Quantitative Score

  • 18.0 [SpecSum]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • SAINT analysis was used to identify SARS-CoV-2 viral-host proximity interactions in human HEK-293 cells and those with a Bayesian FDR =< 0.01 were considered high confidence. Scores are the sum of peptide counts from four mass spec runs with a higher score indicating a higher degree of connectivity between proteins.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP4 STOM
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3801700
NSP4 STOM
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High36.27BioGRID
2792197

Curated By

  • BioGRID