GRB10
Gene Ontology Biological Process
- insulin receptor signaling pathway [ISS, TAS]
- negative regulation of Wnt signaling pathway [IDA]
- negative regulation of glucose import [ISS]
- negative regulation of glycogen biosynthetic process [ISS]
- negative regulation of insulin receptor signaling pathway [ISS]
- positive regulation of phosphorylation [ISS]
- positive regulation of vascular endothelial growth factor receptor signaling pathway [IDA]
- response to insulin [ISS]
Gene Ontology Molecular Function
INSR
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- activation of MAPK activity [IMP]
- activation of protein kinase B activity [IDA]
- activation of protein kinase activity [IMP]
- cellular response to insulin stimulus [IDA]
- glucose homeostasis [IMP]
- heart morphogenesis [IMP]
- insulin receptor signaling pathway [IDA, TAS]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of DNA replication [IMP]
- positive regulation of MAPK cascade [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IC, IDA]
- positive regulation of developmental growth [IMP]
- positive regulation of glucose import [IDA, NAS]
- positive regulation of glycogen biosynthetic process [IDA]
- positive regulation of glycolytic process [IMP]
- positive regulation of mitosis [IMP]
- positive regulation of nitric oxide biosynthetic process [IMP]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of respiratory burst [IDA]
- protein autophosphorylation [IDA, IMP]
- protein heterotetramerization [IDA]
- regulation of embryonic development [IMP]
- regulation of transcription, DNA-templated [IMP]
- signal transduction by phosphorylation [IDA]
- transformation of host cell by virus [IMP]
Gene Ontology Molecular Function- ATP binding [IDA]
- GTP binding [IDA]
- PTB domain binding [IPI]
- insulin binding [IDA, IPI]
- insulin receptor substrate binding [IPI]
- insulin-activated receptor activity [IDA]
- insulin-like growth factor I binding [IPI]
- insulin-like growth factor II binding [IPI]
- insulin-like growth factor receptor binding [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA, IMP]
- receptor signaling protein tyrosine kinase activity [IDA]
- ATP binding [IDA]
- GTP binding [IDA]
- PTB domain binding [IPI]
- insulin binding [IDA, IPI]
- insulin receptor substrate binding [IPI]
- insulin-activated receptor activity [IDA]
- insulin-like growth factor I binding [IPI]
- insulin-like growth factor II binding [IPI]
- insulin-like growth factor receptor binding [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein binding [IPI]
- protein tyrosine kinase activity [IDA, IMP]
- receptor signaling protein tyrosine kinase activity [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Grb-IR: a SH2-domain-containing protein that binds to the insulin receptor and inhibits its function.
To identify potential signaling molecules involved in mediating insulin-induced biological responses, a yeast two-hybrid screen was performed with the cytoplasmic domain of the human insulin receptor (IR) as bait to trap high-affinity interacting proteins encoded by human liver or HeLa cDNA libraries. A SH2-domain-containing protein was identified that binds with high affinity in vitro to the autophosphorylated IR. The mRNA ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| INSR GRB10 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3490566 | |
| GRB10 INSR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| GRB10 INSR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| GRB10 INSR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| GRB10 INSR | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| GRB10 INSR | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| GRB10 INSR | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| GRB10 INSR | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| GRB10 INSR | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| GRB10 INSR | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| GRB10 INSR | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| INSR GRB10 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| INSR GRB10 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| INSR GRB10 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID