An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Transcription-coupled nucleotide excision repair is coordinated by ubiquitin and SUMO in response to ultraviolet irradiation.

Liebelt F, Schimmel J, Verlaan-de Vries M, Klemann E, van Royen ME, van der Weegen Y, Luijsterburg MS, Mullenders LH, Pines A, Vermeulen W, Vertegaal ACO

Cockayne Syndrome (CS) is a severe neurodegenerative and premature aging autosomal-recessive disease, caused by inherited defects in the CSA and CSB genes, leading to defects in transcription-coupled nucleotide excision repair (TC-NER) and consequently hypersensitivity to ultraviolet (UV) irradiation. TC-NER is initiated by lesion-stalled RNA polymerase II, which stabilizes the interaction with the SNF2/SWI2 ATPase CSB to facilitate recruitment of the ... [more]

Nucleic Acids Res. Dec. 10, 2019; 48(1);231-248 [Pubmed: 31722399]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ERCC6

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]DNA binding [IDA]DNA helicase activity [IDA]DNA-dependent ATPase activity [IDA]chromatin binding [IDA]protein C-terminus binding [IPI]protein N-terminus binding [IPI]protein binding [IPI]protein complex binding [IDA]protein tyrosine kinase activator activity [IDA]

Gene Ontology Cellular Component

UQCRC1