FZD7
Gene Ontology Biological Process
- canonical Wnt signaling pathway [IDA, IMP]
- cellular response to retinoic acid [ISS]
- mesenchymal to epithelial transition [IMP]
- negative regulation of cell-substrate adhesion [IMP]
- negative regulation of ectodermal cell fate specification [IMP]
- neuron differentiation [ISS]
- non-canonical Wnt signaling pathway via JNK cascade [IMP]
- positive regulation of JNK cascade [IC]
- positive regulation of epithelial cell proliferation involved in wound healing [IMP]
- positive regulation of phosphorylation [IDA]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- regulation of catenin import into nucleus [IMP]
- regulation of transcription, DNA-templated [IMP]
- stem cell maintenance [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PRNP
Gene Ontology Biological Process
- axon guidance [TAS]
- cellular copper ion homeostasis [NAS]
- metabolic process [TAS]
- negative regulation of T cell receptor signaling pathway [ISS]
- negative regulation of activated T cell proliferation [ISS]
- negative regulation of calcineurin-NFAT signaling cascade [ISS]
- negative regulation of interferon-gamma production [ISS]
- negative regulation of interleukin-17 production [ISS]
- negative regulation of interleukin-2 production [ISS]
- negative regulation of protein phosphorylation [ISS]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISS]
- response to oxidative stress [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
G-protein-coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex biochemical features. To obtain a global view of GPCR-mediated signaling and to identify novel components of their pathways, we used a modified membrane yeast two-hybrid (MYTH) ... [more]
Throughput
- Low Throughput
Additional Notes
- assayed using BRET (bioluminescent resonance energy transfer)
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PRNP FZD7 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
FZD7 PRNP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2827357 |
Curated By
- BioGRID