DNAJC5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GNAS
Gene Ontology Biological Process
- DNA methylation [ISO]
- G-protein coupled receptor signaling pathway [IMP]
- GTP catabolic process [IBA]
- adenylate cyclase-activating G-protein coupled receptor signaling pathway [IDA, ISO]
- adenylate cyclase-activating adrenergic receptor signaling pathway [ISO, ISS]
- adenylate cyclase-activating dopamine receptor signaling pathway [IDA, ISO]
- bone development [ISO]
- calcium ion homeostasis [IMP]
- cartilage development [ISO]
- cellular response to catecholamine stimulus [IDA]
- cellular response to prostaglandin E stimulus [IDA]
- cognition [ISO]
- developmental growth [ISO]
- embryonic cranial skeleton morphogenesis [ISO]
- embryonic hindlimb morphogenesis [ISO]
- endochondral ossification [ISO]
- energy reserve metabolic process [ISO]
- genetic imprinting [ISO]
- hair follicle placode formation [ISO]
- multicellular organism growth [ISO]
- negative regulation of blood pressure [IMP]
- negative regulation of multicellular organism growth [ISO]
- negative regulation of renal sodium excretion [IMP]
- platelet aggregation [ISO]
- positive regulation of Ras GTPase activity [ISO, ISS]
- positive regulation of cAMP biosynthetic process [IMP, ISO]
- positive regulation of cAMP-mediated signaling [ISO, ISS]
- positive regulation of osteoblast differentiation [ISO]
- positive regulation of osteoclast differentiation [ISO]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of sodium ion transport [IMP]
- post-embryonic body morphogenesis [ISO]
- post-embryonic development [ISO]
- regulation of transcription from RNA polymerase II promoter [IMP]
- response to drug [ISO]
- response to parathyroid hormone [ISO]
- sensory perception of chemical stimulus [IBA]
- skeletal system development [ISO]
- skin development [ISO]
- tissue homeostasis [ISO]
Gene Ontology Molecular Function- D1 dopamine receptor binding [IPI]
- G-protein alpha-subunit binding [IPI]
- G-protein beta-subunit binding [IDA]
- G-protein beta/gamma-subunit complex binding [IBA]
- GTP binding [IDA, TAS]
- GTPase activity [IBA]
- alpha-tubulin binding [IDA]
- beta-2 adrenergic receptor binding [IPI]
- corticotropin-releasing hormone receptor 1 binding [IPI]
- insulin-like growth factor receptor binding [IPI]
- ionotropic glutamate receptor binding [IPI]
- mu-type opioid receptor binding [IDA, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- signal transducer activity [IDA, ISO]
- D1 dopamine receptor binding [IPI]
- G-protein alpha-subunit binding [IPI]
- G-protein beta-subunit binding [IDA]
- G-protein beta/gamma-subunit complex binding [IBA]
- GTP binding [IDA, TAS]
- GTPase activity [IBA]
- alpha-tubulin binding [IDA]
- beta-2 adrenergic receptor binding [IPI]
- corticotropin-releasing hormone receptor 1 binding [IPI]
- insulin-like growth factor receptor binding [IPI]
- ionotropic glutamate receptor binding [IPI]
- mu-type opioid receptor binding [IDA, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- signal transducer activity [IDA, ISO]
Gene Ontology Cellular Component
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- dendrite [ISO]
- endosome [IDA]
- extracellular vesicular exosome [ISO]
- heterotrimeric G-protein complex [IDA]
- intrinsic component of membrane [ISO]
- membrane [IDA, ISO]
- membrane raft [IDA]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, TAS]
- ruffle [IDA]
- sarcolemma [IDA]
- trans-Golgi network membrane [ISO]
- vesicle [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Characterization of the G alpha(s) regulator cysteine string protein.
Cysteine string protein (CSP) is an abundant regulated secretory vesicle protein that is composed of a string of cysteine residues, a linker domain, and an N-terminal J domain characteristic of the DnaJ/Hsp40 co-chaperone family. We have shown previously that CSP associates with heterotrimeric GTP-binding proteins (G proteins) and promotes G protein inhibition of N-type Ca2+ channels. To elucidate the mechanisms ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DNAJC5 GNAS | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID