BAIT

SHC1

SHC, SHCA, RP11-307C12.1
SHC (Src homology 2 domain containing) transforming protein 1
Homo sapiens
PREY

PTPN12

P19-PTP, PTP-P19, PTP-PEST, PTPG1
protein tyrosine phosphatase, non-receptor type 12
GO Process (2)
GO Function (4)
GO Component (4)
Mus musculus

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity.

Charest A, Wagner J, Jacob S, McGlade CJ, Tremblay ML

The phosphotyrosine binding (PTB) or phosphotyrosine interaction (PI) domain of the proto-oncoprotein p52SHC binds to an NPXpY consensus sequence found in several growth factor receptors (Kavanaugh, W. M., Turck, C. W., and Williams, L. T. (1994) Science 268, 1177-1179). The amino-terminal region of p52SHC, which includes the PTB/PI domain, has been previously shown to associate with protein-tyrosine phosphatase-PEST (PTP-PEST) in ... [more]

J. Biol. Chem. Apr. 05, 1996; 271(14);8424-9 [Pubmed: 8626541]

Throughput

  • Low Throughput

Curated By

  • BioGRID