BAIT

GABRA1

ECA4, EIEE19, EJM, EJM5
gamma-aminobutyric acid (GABA) A receptor, alpha 1
GO Process (5)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

GABRG3

gamma-aminobutyric acid (GABA) A receptor, gamma 3
GO Process (3)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Detection and binding properties of GABA(A) receptor assembly intermediates.

Klausberger T, Ehya N, Fuchs K, Fuchs T, Ebert V, Sarto I, Sieghart W

Density gradient centrifugation of native and recombinant gamma-aminobutyric acid, type A (GABA(A)) receptors was used to detect assembly intermediates. No such intermediates could be identified in extracts from adult rat brain or from human embryonic kidney (HEK) 293 cells transfected with alpha(1), beta(3), and gamma(2) subunits and cultured at 37 degrees C. However, subunit dimers, trimers, tetramers, and pentamers were ... [more]

J. Biol. Chem. May. 11, 2001; 276(19);16024-32 [Pubmed: 11278514]

Throughput

  • Low Throughput

Curated By

  • BioGRID