Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus.

Barr FA, Preisinger C, Kopajtich R, Koerner R

The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. This structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil proteins, the golgins, and other peripheral membrane components such as GRASP55 and GRASP65. Here we find that TMP21, ... [more]

J. Cell Biol. Dec. 10, 2001; 155(6);885-91 [Pubmed: 11739402]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TMED3 GORASP2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021/pre-pub)	High	0.9713	BioGRID	3284821
GORASP2 TMED3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Barr FA (2001)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GORASP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

TMED3