ENO1
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glycolytic process [TAS]
- negative regulation of cell growth [IDA]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IDA]
- response to virus [IEP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
KIF1B
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.
The complexity of the skeletal muscle and the identification of numerous human disease-causing mutations in its constitutive proteins make it an interesting tissue for proteomic studies aimed at understanding functional relationships of interacting proteins in both health and diseases.We undertook a large-scale study using two-hybrid screens and a human skeletal-muscle cDNA library to establish a proteome-scale map of protein-protein interactions ... [more]
Throughput
- Low Throughput
Additional Notes
- it is not clear whether this immunoprecipitation was performed in lysates from human or mouse cells
Curated By
- BioGRID