Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Publication

A protein phosphatase-1gamma1 isoform selectivity determinant in dendritic spine-associated neurabin.

Carmody LC, Bauman PA, Bass MA, Mavila N, DePaoli-Roach AA, Colbran RJ

Protein phosphatase-1 (PP1) catalytic subunit isoforms interact with diverse proteins, typically containing a canonical (R/K)(V/I)XF motif. Despite sharing approximately 90% amino acid sequence identity, PP1beta and PP1gamma1 have distinct subcellular localizations that may be determined by selective interactions with PP1-binding proteins. Immunoprecipitation studies from brain and muscle extracts demonstrated that PP1gamma1 selectively interacts with spinophilin and neurabin, F-actin-targeting proteins, whereas ... [more]

J. Biol. Chem. May. 21, 2004; 279(21);21714-23 [Pubmed: 15016827]

Throughput

  • Low Throughput

Curated By

  • BioGRID