An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3.

Egana LA, Cuevas RA, Baust TB, Parra LA, Leak RK, Hochendoner S, Pena K, Quiroz M, Hong WC, Dorostkar MM, Janz R, Sitte HH, Torres GE

Uptake through the dopamine transporter (DAT) represents the primary mechanism used to terminate dopaminergic transmission in brain. Although it is well known that dopamine (DA) taken up by the transporter is used to replenish synaptic vesicle stores for subsequent release, the molecular details of this mechanism are not completely understood. Here, we identified the synaptic vesicle protein synaptogyrin-3 as a ... [more]

J. Neurosci. Apr. 08, 2009; 29(14);4592-604 [Pubmed: 19357284]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SLC6A3

Gene Ontology Biological Process

Gene Ontology Molecular Function

dopamine transmembrane transporter activity [IDA]monoamine transmembrane transporter activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

SLC18A2