NOS1
Gene Ontology Biological Process
- aging [IEP]
- arginine catabolic process [IDA]
- behavioral response to cocaine [IMP]
- cellular response to epinephrine stimulus [IMP]
- cellular response to growth factor stimulus [ISO]
- cellular response to mechanical stimulus [IMP]
- exogenous drug catabolic process [ISO]
- female pregnancy [IEP]
- multicellular organismal response to stress [ISO]
- muscle contraction [IBA]
- negative regulation of apoptotic process [IMP]
- negative regulation of blood pressure [IMP]
- negative regulation of calcium ion transport [ISO]
- negative regulation of cell proliferation [IMP]
- negative regulation of cytosolic calcium ion concentration [IMP]
- negative regulation of heart contraction [IMP]
- negative regulation of hydrolase activity [ISO]
- negative regulation of insulin secretion [IMP]
- negative regulation of peptidyl-serine phosphorylation [IMP]
- negative regulation of potassium ion transport [ISO]
- negative regulation of serotonin uptake [ISO]
- negative regulation of vasoconstriction [IMP]
- nitric oxide biosynthetic process [IDA, ISO]
- nitric oxide mediated signal transduction [IBA, IMP]
- peptidyl-cysteine S-nitrosylation [IDA, ISO]
- positive regulation of adrenergic receptor signaling pathway involved in heart process [ISO]
- positive regulation of guanylate cyclase activity [IBA]
- positive regulation of histone acetylation [ISO]
- positive regulation of long-term synaptic potentiation [IMP]
- positive regulation of neuron death [IMP]
- positive regulation of sodium ion transmembrane transport [IMP]
- positive regulation of the force of heart contraction [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- positive regulation of vasodilation [IMP, ISO]
- regulation of heart contraction [IMP]
- regulation of sensory perception of pain [IMP]
- regulation of sodium ion transport [ISO]
- response to activity [IEP]
- response to estrogen [IDA]
- response to ethanol [IEP]
- response to heat [IEP, ISO]
- response to hypoxia [IEP, ISO]
- response to lead ion [IEP]
- response to lipopolysaccharide [IEP]
- response to nicotine [IEP]
- response to nitric oxide [IEP]
- response to nutrient levels [IEP]
- response to organic cyclic compound [IEP]
- response to organonitrogen compound [IEP]
- response to peptide hormone [IMP]
- response to vitamin E [IEP]
- striated muscle contraction [ISO]
Gene Ontology Molecular Function- ATPase binding [IPI]
- FMN binding [IDA, TAS]
- NADP binding [IDA, TAS]
- NADPH-hemoprotein reductase activity [IBA]
- amino acid binding [TAS]
- cadmium ion binding [IDA]
- calmodulin binding [IDA]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA, TAS]
- heme binding [IDA, TAS]
- ion channel binding [IPI]
- nitric-oxide synthase activity [IDA, ISO, TAS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- scaffold protein binding [IPI]
- sodium channel regulator activity [IMP]
- ATPase binding [IPI]
- FMN binding [IDA, TAS]
- NADP binding [IDA, TAS]
- NADPH-hemoprotein reductase activity [IBA]
- amino acid binding [TAS]
- cadmium ion binding [IDA]
- calmodulin binding [IDA]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA, TAS]
- heme binding [IDA, TAS]
- ion channel binding [IPI]
- nitric-oxide synthase activity [IDA, ISO, TAS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- scaffold protein binding [IPI]
- sodium channel regulator activity [IMP]
Gene Ontology Cellular Component
- azurophil granule [IDA]
- cytoplasm [IDA]
- cytoskeleton [ISO]
- cytosol [IDA]
- dendrite [IDA]
- membrane [IDA]
- membrane raft [ISO]
- mitochondrial outer membrane [IDA]
- mitochondrion [IDA]
- nuclear membrane [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- photoreceptor inner segment [IDA]
- plasma membrane [IDA]
- postsynaptic density [IDA]
- protein complex [IDA]
- sarcolemma [IDA, ISO]
- sarcoplasmic reticulum [ISO]
- synapse [IDA, ISO]
- vesicle membrane [IDA]
DLG4
Gene Ontology Biological Process
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [ISS, TAS]
- axon guidance [TAS]
- dendritic spine morphogenesis [ISS]
- establishment of protein localization [IDA]
- learning [TAS]
- negative regulation of receptor internalization [ISS]
- nervous system development [TAS]
- positive regulation of cytosolic calcium ion concentration [ISS]
- positive regulation of excitatory postsynaptic membrane potential [ISS]
- positive regulation of synaptic transmission [ISS]
- protein complex assembly [IDA]
- protein localization to synapse [IDA]
- receptor localization to synapse [ISS]
- regulation of N-methyl-D-aspartate selective glutamate receptor activity [ISS]
- regulation of long-term neuronal synaptic plasticity [ISS]
- signal transduction [TAS]
- synaptic transmission [TAS]
- synaptic vesicle maturation [ISS]
Gene Ontology Molecular Function- D1 dopamine receptor binding [ISS]
- P2Y1 nucleotide receptor binding [ISS]
- PDZ domain binding [ISS]
- acetylcholine receptor binding [ISS]
- beta-1 adrenergic receptor binding [ISS]
- ionotropic glutamate receptor binding [ISS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [ISS]
- protein phosphatase binding [ISS]
- scaffold protein binding [ISS]
- D1 dopamine receptor binding [ISS]
- P2Y1 nucleotide receptor binding [ISS]
- PDZ domain binding [ISS]
- acetylcholine receptor binding [ISS]
- beta-1 adrenergic receptor binding [ISS]
- ionotropic glutamate receptor binding [ISS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [ISS]
- protein phosphatase binding [ISS]
- scaffold protein binding [ISS]
Gene Ontology Cellular Component
- alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex [ISS]
- cell junction [ISS]
- cortical cytoskeleton [IDA]
- cytoplasm [ISS]
- dendrite cytoplasm [ISS]
- dendritic spine [ISS]
- endocytic vesicle membrane [TAS]
- endoplasmic reticulum [ISS]
- excitatory synapse [ISS]
- extrinsic component of cytoplasmic side of plasma membrane [ISS]
- ionotropic glutamate receptor complex [ISS]
- juxtaparanode region of axon [ISS]
- neuron projection terminus [ISS]
- neuron spine [ISS]
- neuronal postsynaptic density [ISS]
- plasma membrane [ISS, TAS]
- postsynaptic density [ISS]
- postsynaptic membrane [IDA]
- synapse [IDA]
- synaptic vesicle [ISS]
- voltage-gated potassium channel complex [ISS]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Splice-isoform specific immunolocalization of neuronal nitric oxide synthase in mouse and rat brain reveals that the PDZ-complex-building nNOSalpha beta-finger is largely exposed to antibodies.
Knock out mice deficient for the splice-isoform alphaalpha of neuronal nitric oxide synthase (nNOSalphaalpha) display residual nitric oxide synthase activity and immunosignal. To attribute this signal to the two minor neuronal nitric oxide synthase splice variants, betabeta and gammagamma, we generated isoform-specific anti-peptide antibodies against the nNOSalphaalpha specific betabeta-finger motif involved in PDZ domain scaffolding and the nNOSbetabeta specific N-terminus. ... [more]
Throughput
- Low Throughput
Additional Notes
- Species of hit is unclear
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DLG4 NOS1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 2811503 |
Curated By
- BioGRID