Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Scaffold role of a mitogen-activated protein kinase phosphatase, SKRP1, for the JNK signaling pathway.

Zama T, Aoki R, Kamimoto T, Inoue K, Ikeda Y, Hagiwara M

Stress-activated protein kinase (SAPK) pathway-regulating phosphatase 1 (SKRP1) has been identified as a member of the mitogen-activated protein kinase (MAPK) phosphatase (MKP) family that interacts physically with the MAPK kinase (MAPKK) MKK7, a c-Jun N-terminal kinase (JNK) activator, and inactivates the MAPK JNK pathway. Although these findings indicated that SKRP1 contributes to the precise regulation of JNK signaling, it remains ... [more]

J. Biol. Chem. Jun. 28, 2002; 277(26);23919-26 [Pubmed: 11959862]

Throughput

  • Low Throughput

Curated By

  • BioGRID