BAIT

HSCB

DNAJC20, HSC20, JAC1, dJ366L4.2, RP3-366L4.2
HscB mitochondrial iron-sulfur cluster co-chaperone
GO Process (1)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

ABCB7

ABC7, ASAT, Atm1p, EST140535
ATP-binding cassette, sub-family B (MDR/TAP), member 7
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.

Maio N, Kim KS, Singh A, Rouault TA

The iron-sulfur (Fe-S) cluster of the Rieske protein, UQCRFS1, is essential for Complex III (CIII) activity, though the mechanism for Fe-S cluster transfer has not previously been elucidated. Recent studies have shown that the co-chaperone HSC20, essential for Fe-S cluster biogenesis of SDHB, directly binds LYRM7, formerly described as a chaperone that stabilizes UQCRFS1 prior to its insertion into CIII. ... [more]

Unknown Apr. 04, 2017; 25(4);945-953.e6 [Pubmed: 28380382]

Throughput

  • High Throughput

Additional Notes

  • endogenous HSC20 was immunoprecipitated from detergent extracts from organellar fractions and associated proteins were identified by mass spectrometry

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ABCB7 HSCB
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-8.0857BioGRID
2455960

Curated By

  • BioGRID