BAIT

HSCB

DNAJC20, HSC20, JAC1, dJ366L4.2, RP3-366L4.2
HscB mitochondrial iron-sulfur cluster co-chaperone
GO Process (1)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

LRPPRC

CLONE-23970, GP130, LRP130, LSFC
leucine-rich pentatricopeptide repeat containing
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.

Maio N, Kim KS, Singh A, Rouault TA

The iron-sulfur (Fe-S) cluster of the Rieske protein, UQCRFS1, is essential for Complex III (CIII) activity, though the mechanism for Fe-S cluster transfer has not previously been elucidated. Recent studies have shown that the co-chaperone HSC20, essential for Fe-S cluster biogenesis of SDHB, directly binds LYRM7, formerly described as a chaperone that stabilizes UQCRFS1 prior to its insertion into CIII. ... [more]

Unknown Apr. 04, 2017; 25(4);945-953.e6 [Pubmed: 28380382]

Throughput

  • High Throughput

Additional Notes

  • endogenous HSC20 was immunoprecipitated from detergent extracts from organellar fractions and associated proteins were identified by mass spectrometry

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HSCB LRPPRC
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High2730BioGRID
2940251

Curated By

  • BioGRID