An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies.

Youn JY, Dunham WH, Hong SJ, Knight JDR, Bashkurov M, Chen GI, Bagci H, Rathod B, MacLeod G, Eng SWM, Angers S, Morris Q, Fabian M, Cote JF, Gingras AC

mRNA processing, transport, translation, and ultimately degradation involve a series of dedicated protein complexes that often assemble into large membraneless structures such as stress granules (SGs) and processing bodies (PBs). Here, systematic in vivo proximity-dependent biotinylation (BioID) analysis of 119 human proteins associated with different aspects of mRNA biology uncovers 7424 unique proximity interactions with 1,792 proteins. Classical bait-prey analysis reveals ... [more]

Mol. Cell Dec. 01, 2017; 69(3);517-532.e11 [Pubmed: 29395067]

Throughput

High Throughput

Additional Notes

BioID
High confidence interactions had a SAINTexpress score or AvgP >= 0.95.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PABPC1 ATXN2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Marcon E (2014)	High	17.1786	BioGRID	2947637
PABPC1 ATXN2	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Kozlov G (2010)	Low	-	BioGRID	-
PABPC1 ATXN2	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2022)	High	-	BioGRID	3437550
ATXN2 PABPC1	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	0.4393	BioGRID	1266034
PABPC1 ATXN2	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Youn JY (2018)	Low	-	BioGRID	2815332
PABPC1 ATXN2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Ralser M (2005)	Low	-	BioGRID	-
ATXN2 PABPC1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Ralser M (2005)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ATXN2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [NAS]epidermal growth factor receptor binding [IPI]poly(A) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

PABPC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]poly(A) binding [IDA, TAS]poly(U) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]translation activator activity [TAS]