An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies.

Youn JY, Dunham WH, Hong SJ, Knight JDR, Bashkurov M, Chen GI, Bagci H, Rathod B, MacLeod G, Eng SWM, Angers S, Morris Q, Fabian M, Cote JF, Gingras AC

mRNA processing, transport, translation, and ultimately degradation involve a series of dedicated protein complexes that often assemble into large membraneless structures such as stress granules (SGs) and processing bodies (PBs). Here, systematic in vivo proximity-dependent biotinylation (BioID) analysis of 119 human proteins associated with different aspects of mRNA biology uncovers 7424 unique proximity interactions with 1,792 proteins. Classical bait-prey analysis reveals ... [more]

Mol. Cell Dec. 01, 2017; 69(3);517-532.e11 [Pubmed: 29395067]

Throughput

High Throughput

Additional Notes

BioID
High confidence interactions had a SAINTexpress score or AvgP >= 0.95.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ELAVL1 PTBP2	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Abdelmohsen K (2009)	High	-	BioGRID	664075
ELAVL1 PTBP2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hegele A (2012)	High	-	BioGRID	-
PTBP2 ELAVL1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hegele A (2012)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ELAVL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

AU-rich element binding [IDA]RNA binding [IDA]double-stranded RNA binding [IDA]mRNA 3'-UTR AU-rich region binding [IDA]mRNA 3'-UTR binding [ISS]mRNA binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]

Gene Ontology Cellular Component

PTBP2