A genetic interaction is inferred when mutations or deletions in separate genes, at least one of which is hemizygous, cause a minimal phenotype alone but result in lethality when combined in the same cell under a given condition.

Publication

Modeling complex genetic interactions in a simple eukaryotic genome: actin displays a rich spectrum of complex haploinsufficiencies.

Haarer B, Viggiano S, Hibbs MA, Troyanskaya OG, Amberg DC

Multigenic influences are major contributors to human genetic disorders. Since humans are highly polymorphic, there are a high number of possible detrimental, multiallelic gene pairs. The actin cytoskeleton of yeast was used to determine the potential for deleterious bigenic interactions; approximately 4800 complex hemizygote strains were constructed between an actin-null allele and the nonessential gene deletion collection. We found 208 ... [more]

Genes Dev. Jan. 15, 2007; 21(2);148-59 [Pubmed: 17167106]

Throughput

High Throughput

Ontology Terms

phenotype: haploinsufficient (APO:0000152)

Additional Notes

these complex haploinsufficient interactions with the act1-null heterozygote are sick

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MRPL35 ACT1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3187	BioGRID	2036196

Curated By

BioGRID

Interaction Summary

ACT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone acetyltransferase activity [IDA]structural constituent of cytoskeleton [IDA]

Gene Ontology Cellular Component

MRPL35