BAIT

ACT1

ABY1, END7, actin, L000000025, YFL039C
Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
Saccharomyces cerevisiae (S288c)
PREY

GIS2

L000004862, YNL255C
Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2
GO Process (1)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Haploinsufficiency

A genetic interaction is inferred when mutations or deletions in separate genes, at least one of which is hemizygous, cause a minimal phenotype alone but result in lethality when combined in the same cell under a given condition.

Publication

Modeling complex genetic interactions in a simple eukaryotic genome: actin displays a rich spectrum of complex haploinsufficiencies.

Haarer B, Viggiano S, Hibbs MA, Troyanskaya OG, Amberg DC

Multigenic influences are major contributors to human genetic disorders. Since humans are highly polymorphic, there are a high number of possible detrimental, multiallelic gene pairs. The actin cytoskeleton of yeast was used to determine the potential for deleterious bigenic interactions; approximately 4800 complex hemizygote strains were constructed between an actin-null allele and the nonessential gene deletion collection. We found 208 ... [more]

Genes Dev. Jan. 15, 2007; 21(2);148-59 [Pubmed: 17167106]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: haploinsufficient (APO:0000152)

Additional Notes

  • these complex haploinsufficient interactions with the act1-null heterozygote are sick

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GIS2 ACT1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2913BioGRID
2442875

Curated By

  • BioGRID