An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The acidic region of the factor VIII light chain and the C2 domain together form the high affinity binding site for von willebrand factor.

Saenko EL, Scandella D

A binding site for von Willebrand factor (vWf) was previously localized to the carboxyl terminus of the C2 domain of the light chain (LCh) of factor VIII (fVIII). The acidic region of the LCh, residues 1649-1689, also controls fVIII.vWf binding by an unknown mechanism. Although anti-acidic region monoclonal antibodies prevent formation of the fVIII.vWf complex, the direct involvement of the ... [more]

J. Biol. Chem. Jul. 18, 1997; 272(29);18007-14 [Pubmed: 9218428]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

VWF

Gene Ontology Biological Process

Gene Ontology Molecular Function

chaperone binding [IDA]collagen binding [IDA]glycoprotein binding [IDA]identical protein binding [IPI]immunoglobulin binding [IDA]integrin binding [IPI]protease binding [IDA, IPI]protein N-terminus binding [IPI]protein binding [IPI]protein homodimerization activity [IDA]

Gene Ontology Cellular Component

F8