An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies.

Youn JY, Dunham WH, Hong SJ, Knight JDR, Bashkurov M, Chen GI, Bagci H, Rathod B, MacLeod G, Eng SWM, Angers S, Morris Q, Fabian M, Cote JF, Gingras AC

mRNA processing, transport, translation, and ultimately degradation involve a series of dedicated protein complexes that often assemble into large membraneless structures such as stress granules (SGs) and processing bodies (PBs). Here, systematic in vivo proximity-dependent biotinylation (BioID) analysis of 119 human proteins associated with different aspects of mRNA biology uncovers 7424 unique proximity interactions with 1,792 proteins. Classical bait-prey analysis reveals ... [more]

Mol. Cell Dec. 01, 2017; 69(3);517-532.e11 [Pubmed: 29395067]

Throughput

Low Throughput

Additional Notes

BioID
High confidence interactions had a SAINTexpress score or AvgP >= 0.95.

Curated By

BioGRID

Interaction Summary

EIF4A3

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]ATP-dependent RNA helicase activity [IDA]mRNA binding [IDA]poly(A) RNA binding [IDA]poly(A) binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

WDR36