BAIT

HMGB1

HMG1, HMG3, SBP-1, RP11-550P23.1
high mobility group box 1
GO Process (22)
GO Function (13)
GO Component (7)
Homo sapiens
PREY

HOXB3

HOX2, HOX2G, Hox-2.7
homeobox B3
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation.

Zappavigna V, Falciola L, Helmer-Citterich M, Mavilio F, Bianchi ME

High mobility group protein 1 (HMG1) is a non-histone, chromatin-associated nuclear protein with a proposed role in the regulation of eukaryotic gene expression. We show that HMG1 interacts with proteins encoded by the HOX gene family by establishing protein-protein contacts between the HMG box domains and the HOX homeodomain. The functional role of these interactions was studied using the transcriptional ... [more]

EMBO J. Sep. 16, 1996; 15(18);4981-91 [Pubmed: 8890171]

Throughput

  • Low Throughput

Curated By

  • BioGRID