HTR1A
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IMP]
- adenylate cyclase-inhibiting serotonin receptor signaling pathway [IDA]
- behavioral fear response [ISS]
- exploration behavior [ISS]
- positive regulation of cell proliferation [TAS]
- regulation of dopamine metabolic process [ISS]
- regulation of serotonin secretion [ISS]
- serotonin metabolic process [ISS]
- serotonin receptor signaling pathway [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GPR26
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Oligomerization of G-protein-coupled receptors shown by selective co-immunoprecipitation.
Recent studies have shown that G-protein-coupled receptors (GPCRs) can assemble as high molecular weight homo- and hetero-oligomeric complexes. This can result in altered receptor-ligand binding, signaling, or intracellular trafficking. We have co-transfected HEK-293 cells with differentially epitope-tagged GPCRs from different subfamilies and determined whether oligomeric complexes were formed by co-immunoprecipitation and immunoblot analysis. This gave the surprising result that the ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID