CNOT1
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- RNA phosphodiester bond hydrolysis, exonucleolytic [IDA]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- negative regulation of intracellular estrogen receptor signaling pathway [IDA]
- negative regulation of retinoic acid receptor signaling pathway [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of cytoplasmic mRNA processing body assembly [IDA]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMP]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [IMP]
- regulation of stem cell maintenance [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CNOT2
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- RNA phosphodiester bond hydrolysis, exonucleolytic [IMP]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- negative regulation of intracellular estrogen receptor signaling pathway [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of cytoplasmic mRNA processing body assembly [IMP]
- regulation of stem cell maintenance [IMP]
- regulation of transcription from RNA polymerase II promoter [NAS]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies.
mRNA processing, transport, translation, and ultimately degradation involve a series of dedicated protein complexes that often assemble into large membraneless structures such as stress granules (SGs) and processing bodies (PBs). Here, systematic in vivo proximity-dependent biotinylation (BioID) analysis of 119 human proteins associated with different aspects of mRNA biology uncovers 7424 unique proximity interactions with 1,792 proteins. Classical bait-prey analysis reveals ... [more]
Throughput
- Low Throughput
Additional Notes
- BioID
- High confidence interactions had a SAINTexpress score or AvgP >= 0.95.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CNOT2 CNOT1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| CNOT2 CNOT1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1451709 | |
| CNOT1 CNOT2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1445839 | |
| CNOT1 CNOT2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| CNOT1 CNOT2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CNOT1 CNOT2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CNOT2 CNOT1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CNOT1 CNOT2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3429496 | |
| CNOT2 CNOT1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 2812692 | |
| CNOT1 CNOT2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID