SPDEF
Gene Ontology Biological Process
Gene Ontology Molecular Function
NKX3-1
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- androgen receptor signaling pathway [IDA]
- branching involved in prostate gland morphogenesis [ISS]
- branching morphogenesis of an epithelial tube [ISS]
- cellular response to drug [IEP]
- cellular response to hypoxia [IDA]
- cellular response to interleukin-1 [IEP]
- cellular response to steroid hormone stimulus [IDA]
- cellular response to tumor necrosis factor [IEP]
- epithelial cell proliferation involved in salivary gland morphogenesis [ISS]
- mitotic cell cycle arrest [IDA]
- multicellular organismal development [TAS]
- negative regulation of cell proliferation [IDA]
- negative regulation of epithelial cell proliferation [ISS]
- negative regulation of epithelial cell proliferation involved in prostate gland development [ISS]
- negative regulation of estrogen receptor binding [IDA]
- negative regulation of gene expression [IDA]
- negative regulation of insulin-like growth factor receptor signaling pathway [ISS]
- negative regulation of mitotic cell cycle [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation of androgen secretion [IDA]
- positive regulation of apoptotic signaling pathway [IDA]
- positive regulation of cell death [IDA]
- positive regulation of cell division [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of gene expression [IDA, IMP]
- positive regulation of intrinsic apoptotic signaling pathway [IDA]
- positive regulation of mitotic cell cycle [IMP]
- positive regulation of phosphatidylinositol 3-kinase signaling [IDA]
- positive regulation of protein kinase activity [IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of response to DNA damage stimulus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IMP, ISS]
- protein kinase B signaling [IMP]
- regulation of transcription, DNA-templated [NAS]
- response to testosterone [ISS]
- salivary gland development [ISS]
- steroid hormone mediated signaling pathway [IDA]
Gene Ontology Molecular Function- androgen receptor activity [IDA]
- core promoter binding [IDA]
- cysteine-type endopeptidase activator activity involved in apoptotic process [IDA]
- estrogen receptor activity [IDA]
- estrogen receptor binding [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein kinase activator activity [IDA]
- protein self-association [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, NAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transcription regulatory region sequence-specific DNA binding [IDA]
- androgen receptor activity [IDA]
- core promoter binding [IDA]
- cysteine-type endopeptidase activator activity involved in apoptotic process [IDA]
- estrogen receptor activity [IDA]
- estrogen receptor binding [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein kinase activator activity [IDA]
- protein self-association [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, NAS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transcription regulatory region sequence-specific DNA binding [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter.
The NKX-3.1 homeobox gene maps to human chromosome 8p21, a region that undergoes frequent loss of heterozygosity in prostate tumors. Loss of Nkx-3.1 function in mice leads to epithelial overgrowth. To further elucidate the molecular basis of NKX-3.1 function, a genetic screen for proteins that interact with NKX-3.1 was performed. Prostate-derived Ets factor (PDEF) was identified as a potential partner ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SPDEF NKX3-1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| NKX3-1 SPDEF | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID